Figure 5.

The effects of disrupting LCMT1 expression in HeLa cells on the holoenzyme composition of PP2A, PP4, and PP6. (A) Western blots of wild-type (WT) HeLa and a hypomorph (HM) Hela clone probed for LCMT1, methylated PP2Acα/β, and total PP2Acα/β. (B) Western blots were quantified, and individual data points are shown for LCMT1, methylated PP2Acα/β, and total PP2Acα/β intensities normalized to total protein loading. Error bars represent the standard deviation of four replicates. Mann–Whitney test was performed *p < 0.05, ns – not significant. The log₂ HM/WT ratio of the abundance of the catalytic subunits (C), PP2A subunits (D), PP4 subunits (E), and PP6 subunits (F) bound to PIBs. (G) Scatterplot of the log₂ HM/WT ratio of PP2A, PP4, and PP6 subunits PIB binding and normalized to the respective catalytic subunit in HEK293T versus Hela cells. PP2A subunits are labeled in purple, PP4 subunits are in blue, and PP6 subunits are in green. The scatterplot in the upper right depicts catalytic subunit PIB binding in both cell types. *p < 0.05.