TABLE 1.
Yeast strains
| Strain | Relevant genotypea | Source or construction |
|---|---|---|
| MS71 | LEU2 | 52 |
| EAS63a | a ade2-100 ADE5 LEU2 | E. A. Sia |
| RJK48 | a ade2-100 ADE5 | Described in Materials and Methods |
| HM6 | LEU2 rad52Δ::hisG-URA3-hisG | H. Moore |
| GCY140 | LEU2 msh3Δ | 52 |
| RJK341 | LEU2 kanMX-GAL1-(3×HA)-POL3 | Described in Materials and Methods |
| RJK368-4 | LEU2 kanMX-GAL1-(3×HA)-POL3 pep4::URA3 | Described in Materials and Methods |
| MS71-pol3-t | LEU2 pol3-t | 22 |
| JED213-30 | LEU2 (3×HA)-POL3 | Described in Materials and Methods |
| RJK394 | LEU2 (3×HA)-POL3 pep4::URA3 | Described in Materials and Methods |
| RJK366-5 | LEU2 pep4::URA3 | Described in Materials and Methods |
| RJK370 | a rad52Δ::hisG-URA3-hisG | Spore from RJK364b |
| RJK396 | a LEU2 | Spore from RJK372b |
| RJK397 | rad52Δ::hisG-URA3-hisG | Spore from RJK372b |
| RJK398 | kanMX-GAL1-(3×HA)-POL3 | Spore from RJK372b |
| RJK399 | a LEU2 rad52Δ::hisG-URA3-hisG kanMX-GAL1-(3×HA)-POL3 | Spore from RJK372b |
| LS31 | rev3Δ::kanMX | J. Gerton |
| EAS102 | LEU2 rad1Δ | E. A. Sia |
| RJK67-5 | a ade2-100 ADE5 + pBL304[CEN4/URA3/POL3] | RJK48 transformed with pBL304 |
| RJK78-3 | a ade2-100 ADE5 + pBK100 [CEN4/LEU2/POL3] | Described in Materials and Methods |
| RJK122-3 | a ade2-100 ADE5 pol3::kanMX + pBL304 | Described in Materials and Methods |
| RJK160-1 | a ade2-100 ADE5 pol3::kanMX + pBK100 | Plasmid shuffle of RJK122-3c |
| RJK158-3 | a ade2-100 ADE5 pol3::kanMX + pBK101 [CEN4/LEU2/pol3-ts1] | Plasmid shuffle of RJK122-3c |
| RJK185-1 | a ade2-100 ADE5 pol3::kanMX + pBK102 [CEN4/LEU2/pol3-ts8] | Plasmid shuffle of RJK122-3c |
| RJK187-1 | a ade2-100 ADE5 pol3::kanMX + pBK103 [CEN4/LEU2/pol3-ts11] | Plasmid shuffle of RJK122-3c |
| RJK218-2 | a ade2-100 ADE5 pol3::kanMX + pBK104 [CEN4/LEU2/pol3-ts15] | Plasmid shuffle of RJK122-3c |
| RJK223-1 | a ade2-100 ADE5 pol3::kanMX + pBK105 [CEN4/LEU2/pol3-ts26] | Plasmid shuffle of RJK122-3c |
| RJK286-1 | a ade2-100 ADE5 pol3::kanMX + pBK106 [CEN4/LEU2/pol3-ts28] | Plasmid shuffle of RJK122-3c |
| RJK287-1 | a ade2-100 ADE5 pol3::kanMX + pBK107 [CEN4/LEU2/pol3-ts29] | Plasmid shuffle of RJK122-3c |
| RJK288 | a ade2-100 ADE5 pol3::kanMX rad52Δ::hisG-URA3-hisG + pBK101 | Spore from RJK272b |
| RJK334 | a ade2-100 ADE5 pol3::kanMX rad52Δ::hisG-URA3-hisG + pBK100 | Spore from RJK310b |
| RJK335 | a ade2-100 ADE5 pol3::kanMX rad52Δ::hisG-URA3-hisG + pBK100 | Spore from RJK310b |
| RJK357 | a ade2-100 ADE5 pol3::kanMX msh3Δ + pBK100 | Spore from RJK344b |
| RJK358 | a ade2-100 ADE5 pol3::kanMX msh3Δ + pBK101 | Spore from RJK345b |
| RJK363 | a ade2-100 ADE5 pol3::kanMX msh3Δ rad52Δ::hisG-URA3-hisG + pBK101 | Spore from RJK345b |
| RJK381 | a ade2-100 ADE5 pol3::kanMX msh3Δ rad52Δ::hisG-URA3-hisG + pBK101 | Spore from RJK373b |
| RJK379 | a ade2-100 ADE5 pol3::kanMX msh3Δ rad52Δ::hisG + pBK101 | 5-FOAr isolate of RJK363 |
| RJK242-1 | a ade2-100 ADE5 pol3::kanMX + pBK100 + pSH44 | RJK160-1 transformed with pSH44 |
| RJK166-4 | a ade2-100 ADE5 pol3::kanMX + pBK101 + pSH44 | RJK158-3 transformed with pSH44 |
| RJK202-3 | a ade2-100 ADE5 pol3::kanMX + pBK103 + pSH44 | RJK187-1 transformed with pSH44 |
| RJK232-5 | a ade2-100 ADE5 pol3::kanMX + pBK105 + pSH44 | RJK223-1 transformed with pSH44 |
| LS38-1 | a ade2-100 ADE5 pol3::kanMX msh3Δ + pBK100 + pSH44 | RJK357 transformed with pSH44 |
| LS40-1 | a ade2-100 ADE5 pol3::kanMX msh3Δ + pBK101 + pSH44 | RJK358 transformed with pSH44 |
| RJK380-1 | a ade2-100 ADE5 pol3::kanMX msh3Δ rad52Δ::hisG + pBK101 + pSH44 | RJK379 transformed with pSH44 |
a
All strains are isogenic with AMY125 (α trp1-289 his7-2 ura3-52 leu2-3 ade5-1 [51]) except for the changes introduced by transformation. Only alterations from the AMY125 genotype are shown.
b
Diploids referred to were constructed by the following crosses: RJK310 (RJK160-1 × HM6), RJK344 (RJK160-1 × GCY140), RJK345 (RJK288 × GCY140), RJK272 (RJK158-3 × HM6), RJK373 (RJK358 × HM6), RJK364 (LS31 × RJK288), and RJK372 (RJK341 × RJK370).
c
These strains were generated by transforming RJK122-3 with mutagenized pBK100, followed by screening for derivatives of the transformed strain that lost the pBL304 plasmid.