TABLE 6.
Mutational spectra at CAN1 in strains with POL3 nonsense mutations
| Strain genotypea | Type and frequency of mutation
|
|||
|---|---|---|---|---|
| Base substitutions | Alterations within simple repeats | Deletions (bp) flanked by direct repeats | Other | |
| pol3-ts8 | ||||
| Total | 22% (2/9) | 11% (1/9) | 56% (5/9) | 11% (1/9) |
| C to A (1/9) | (AT)2 to (AT)1 (1/9) | 18 (1/9) | Telomere addition (1/9)b | |
| A to T (1/9) | 27 (1/9) | |||
| 30 (1/9) | ||||
| 38 (1/9) | ||||
| 662 (1/9) | ||||
| pol3-ts15 | ||||
| Total | 33% (3/9) | 0% (0/9) | 56% (5/9) | 11% (1/9) |
| C to T (1/9) | 16 (2/9) | 17-bp duplication (1/9)c | ||
| G to A (1/9) | 18 (1/9) | |||
| C to G (1/9) | 268 (1/9) | |||
| 426 (1/9) | ||||
a
Strain names are as follows: RJK185-1 (pol3-ts8) and RJK218-2 (pol3-ts15).
b
Telomere addition. This isolate contains a loss of chromosome V DNA up to nucleotide 34831 (as defined by Stanford Saccharomyces Genome Database) and the addition of a telomere of indeterminate length. The DNA sequence at this breakpoint is as follows (capital letters represent chromosome V DNA, and lowercase letters represent the new telomeric sequence): GTGGAGGGTGTTGTTGTGGAGTgggtgtggtgtgtg.
c
All duplications had short direct repeats flanking the duplicated segment, similar to those observed in rad27 strains (54).