TABLE 7.
Effect of POL3 expression level on mutational spectra at CAN1 in yeast strain RJK341
| Growth media | Type and frequency of mutation
|
|||
|---|---|---|---|---|
| Base substitutions | Alterations within simple repeats | Deletions (bp) flanked by direct repeats | Other | |
| YPR + 0.05% galactose | ||||
| Total | 84% (16/19) | 0% (0/19) | 5% (1/19) | 11% (2/19) |
| G to T (4/19) | 206 (1/19) | ATA to TT (1/19) | ||
| G to A (3/19) | TT to ATA (1/19) | |||
| C to T (3/19) | ||||
| C to A (2/19) | ||||
| A to T (1/19) | ||||
| T to C (1/19) | ||||
| T to G (1/19) | ||||
| T to A (1/19) | ||||
| YPR + 0.005% galactose | ||||
| Total | 32% (6/19) | 5% (1/19) | 58% (11/19) | 5% (1/19) |
| G to C (2/19) | T5 to T4 (1/19) | 15 (1/19) | 1-bp deletion (1/19) | |
| T to A (2/19) | 16 (2/19) | |||
| C to G (1/19) | 18 (2/19) | |||
| C to T (1/19) | 24 (1/19) | |||
| 39 (2/19) | ||||
| 49 (1/19) | ||||
| 53 (2/19) | ||||
| YPR | ||||
| Total | 40% (8/20) | 5% (1/20) | 45% (9/20) | 10% (2/20) |
| G to C (3/20) | A5 to A6 (1/20) | 8 (2/20) | GTA to TTT (1/20) | |
| T to A (2/20) | 16 (2/20) | Two base substitutions: A to C and G to C (1/20) | ||
| C to G (1/20) | 17 (1/20) | |||
| A to T (1/20) | 29 (1/20) | |||
| C to A (1/20) | 59 (1/20) | |||
| 81 (1/20) | ||||
| 1,077 (1/20) | ||||