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Content of the vials of the PCR reaction
| Reagent | Volume | Final Concentration |
|---|---|---|
| SYBER Master Mix (2X) | 10 μl | 1x |
| Forward Primer (10 μl) | 0.6 μl | 0.3 μM |
| Reverse Primer (10 μl) | 0.6 μl | 0.3 μM |
| Rox Reference Dye (50X) | 0.4 μl for ABI Step One/Plus | 1x |
| Template | 2 μl | < 100 ng |
| DDW | 7.2 μl | |
| Total | 20 μl |
