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. 2021 Nov 23;14:5333–5334. doi: 10.2147/OTT.S350023

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© 2021 Hu et al.

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Dehydrocorydaline inhibits cell migration and invasion in melanoma cells. (A) Cell migration rate detected by wound-healing assay of A375 and MV3 cells after treating with DMSO or 40 μM DHC for the indicated time. Scale bar, 100 μm. (B) The effect of 40 μM DHC on the wound closure in A375 and MV3 cells. (C) The effect of transwell migration assays in A375 and MV3 cells after treating with DMSO or 40 μM DHC for 24 h. Scale bar, 100 μm. Migration rates were normalized by proliferation. (D) The effect of transwell invasion assays in A375 and MV3 cells after treating with DMSO or 40 μM DHC for 72 h. Scale bar, 100 μm. Invasion rates were normalized by proliferation. (E and F) Western blot analysis of the metastasis-related protein levels in A375 and MV3 cells, respectively. Protein levels were calculated based on the grayscale value of protein bands and normalized with the grayscale value of GAPDH bands. Cells were treated with the indicated concentrations (0, 20, 40, 80 μM) of DHC for 48 h or with 40 μM DHC treatment for indicated times (0, 24, 48, 72 h) of DHC; GAPDH was used as a control. All data are shown as the mean ± SD. A two-tailed unpaired Student’s t-test was carried out. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Abbreviations: DHC, dehydrocorydaline; DMSO, dimethyl sulfoxide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.