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. 2021 Nov 24;47(1):21. doi: 10.3892/or.2021.8232

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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Figure 2. — FGD5-AS1 knockdown suppresses pancreatic cancer cell proliferation, migration and invasion. (A) FGD5-AS1 siRNA or control siRNA was transfected into SW1990 cells to construct the knockdown model of FGD5-AS1, and RT-qPCR was employed to detect the interference efficiency of siRNAs. (B) An MTT assay was employed to detect the effect of knockdown of FGD5-AS1 on SW1990 cell proliferation. (C) Transwell assays were utilized to detect the effect of knockdown of FGD5-AS1 on SW1990 cell migration and invasion. Scale bar, 50 µm. All of the experiments were performed in triplicate. **P<0.01 and ***P<0.001. FGD5-AS1, FGD5 antisense RNA 1; si-, small interfering; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control.