Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 24;47(1):21. doi: 10.3892/or.2021.8232

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

PMC Copyright notice

Figure 3. — FGD5-AS1 directly targets miR-577. (A) Bioinformatics analysis was carried out to predict the binding sites between miR-577 and FGD5-AS1, and FGD5-AS1-WT and FGD5-AS1-MUT luciferase reporter gene vectors were constructed. (B) A dual-luciferase reporter assay was utilized to determine the binding site between FGD5-AS1 and miR-577. (C) Pearson's correlation analysis was employed to analyze the correlation between FGD5-AS1 expression and miR-577 expression in pancreatic cancer tissues. (D) An RNA pull-down assay was employed to validate the direct interaction between miR-577 and FGD5-AS1. (E) A RIP assay was carried out to confirm that the complex containing miR-577 and FGD5-AS1 in SW1990 cells was immunoprecipitated by anti-Ago2. All of the experiments were performed in triplicate. ***P<0.001. FGD5-AS1, FGD5 antisense RNA 1; miR, microRNA; WT, wild-type; MUT, mutant; RIP, RNA immunoprecipitation.