Table I.
Primer sequences for RT-qPCR.
| Genes | Primer sequences (5′-3′) |
|---|---|
| FGD5-AS1 | F: AGAAGCGGAGGGGTGAAAAT |
| R: CCGCCTTATAGTTGGCCCTC | |
| LRP6 | F: AGGCACTTACTTCCCTGCAA |
| R: GGGCACAGGTTCTGAATCAT | |
| β-catenin | F: GACATCAACGTGGTGACCTG |
| R: GCTGGCTCTGTGATTTCCTC | |
| GAPDH | F: GGAGCGAGATCCCTCCAAAAT |
| R: GGCTGTTGTCATACTTCTCATGG | |
| miR-577 | F: TGCGGTAGATAAAATATTGG |
| R: CCAGTGCAGGGTCCGAGGT | |
| U6 | F: GCTCGCTTCGGCAGCACA |
| R: GAGGTATTCGCACCAGAGGA |
RT-qPCR, reverse transcription quantitative polymerase chain reaction; LRP6, low-density lipoprotein receptor-related protein 6; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; R, reverse.