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. 2021 Nov 8;9:738081. doi: 10.3389/fbioe.2021.738081

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Copyright © 2021 Liu, Zhou, Chen, Lv, Huang, Peng, Wu, Chen, Tang, Guo, Wang, Zhang, Liu, Yang, Yu and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HUVECs produced GDNF that promoted HepLPC maturation by phosphorylation of Met. (A) Gene expression was analyzed by qRT-PCR. GDNF, GDNF receptors (Ret, GARF1), and HGF were analyzed. Data are normalized to HepLPCs. (B) Immunofluorescence confirmed that the expression of the GDNF receptor, Ret, increased after co-culture. (C) Quantitative analysis of HGF secretion in supernatants. Data are normalized to HepLPCs (n = 4 per group). (D) The expression of different batches of Met, p-Met, and GDNF after co-culture was assessed using western blots (n = 3 per group). (E) Western blots were used to detect GNDF, Met, and p-Met in HepLPCs and HepLPCs that overexpressed GDNF (n = 3 per group). (F) Immunofluorescent staining analyses of the expression of hepatic function markers (ALB, AAT) in HepLPCs and HepLPCs that overexpressed GDNF. Nuclei were counterstained with DAPI.