Figure 6.

Flagella with swelling exhibit a higher number of rosette-like formations. (A) Representative general and detailed views of Trichomonas vaginalis anterior flagella (AF) without and with extra-axonemal structures (EAS) obtained by TEM. Many rosette-like formations (white arrows) are seen in the flagella with swelling (black arrows). As, axostyle. (B) Quantification of the number of rosettes/µm². The columns represent the average number of rosettes/µm² ± standard deviation (SD) of three independent experiments. Fifty flagella with or without swellings per sample were randomly counted using TEM. The dots indicate the values obtained for each flagellum. Flagella with EAS show a higher number of rosettes/µm² than those flagella without EAS. ****p < 0.0001 compared with the “no-EAS” group using non-parametric t-test (Mann–Whitney test).