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. Author manuscript; available in PMC: 2021 Nov 30.
Published in final edited form as: J Proteome Res. 2017 Jan 9;16(2):538–549. doi: 10.1021/acs.jproteome.6b00672

Figure 2. Hierarchical clustering of differentially expressed proteins and phosphoproteins.

Figure 2.

A) Proteome data was filtered to contain proteins quantified by 2 or more peptides, with a CV of <30% for triplicate QC injections, and p<0.1 for a paired t-test w/ FDR correction. The remaining 874 proteins were normalized using Z-scoring and visualized by two-dimensional hierarchical clustering (Ward method). Cluster 7 (green) is composed predominantly of proteins expressed in multiciliated airway cells, and clusters 10–12 (yellow) are composed predominantly of proteins expressed during squamous differentiation (see Results). B) Phosphopeptides were filtered by CV <30% and p<0.1 and visualized as in (C). Data was analyzing using JMP (SAS, Cary, NC).