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. 2021 Nov 9;12:780910. doi: 10.3389/fimmu.2021.780910

Figure 2.

Figure 2

Neutrophils in the oral cavity are decreased in HIV-infected individuals and have low surface Gal-9. (A) Representative flow cytometry plots for CD15+ neutrophil identification in the oral wash. (B) Cumulative data of percentages of CD15+ cells in the oral washes of HCs compared to HIV-infected individuals. (C) Representative flow cytometry plots, and (D) cumulative data of neutrophils in oral washes of different HIV-infected individuals (ART-naïve, on ART with low CD4+ T cell count (< 200 cells/ul) or high CD4+ T cell count (> 200 cells/ul), and long-term non-progressor (LTNP) compared to HCs. (E) Representative flow cytometry plots, and (F) cumulative data of Gal-9 surface expression in neutrophils from the blood and oral washes of HIV and healthy individuals. (G) Representative histogram plots, and (H) cumulative data of Gal-9 expression measured by the Mean Fluorescence Intensity (MFI) on blood and oral neutrophils of HIV-infected and healthy individuals. (I) Cumulative data of soluble Gal-9 in the plasma and saliva of HIV-infected and healthy individuals as measured by ELISA. (J) Cumulative data of a positive correlation between the saliva Gal-9 with the saliva IL-6 (Spearman correlation, r = 0.54, P = 0.008). (K) Representative histogram plots, and (L) cumulative data of apoptosis in oral neutrophils in regard to Gal-9 expression measured by Annexin V assay. ns, no significant.