FIGURE 6.

Overexpression of circ-ATXN2 enhanced apoptosis in rat adipose-derived stem cells (ASCs). Cell apoptosis was determined by Annexin V-APC/PI counterstain followed by flow cytometry. (A) Apoptosis, detected using flow cytometry, showed the apoptotic cell rate in vector-GFP or circ-ATXN2-GFP-expressing cells. ASCs were without treated by CoCl₂. (B) Data from experiments performed at least in triplicate are presented, showing the percentage of total apoptosis. (C) Apoptosis assay detected by flow cytometry showed the apoptotic cell rate in vector-GFP or circ-ATXN2-GFP cells. ASCs were treated by CoCl₂ (500 μM, 24 h). (D) Data from experiments performed at least in triplicate are presented, showing the percentage of total apoptosis percentage. Results were presented as means ± SEM, by Student’s t test, n = 3; **p < 0.01, NS: no significance vs vector-GFP-expressing cells.