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. 2021 Nov 9;6(11):3994–4000. doi: 10.1021/acssensors.1c01369

Figure 2.

Figure 2

Recombinant FRET-based TAv biosensors for pH and K+ remain functional upon immobilization on biotinylated glass slides. (a) Illustration of a coupled biosensor to a biotinylated glass surface. (b) Representative response of glass-coupled TAv–pH-Lemon perfused with buffers with different pH values after lyophilization and storage for 24 h. (c) Schematic drawing and representative widefield images of the application of a 2 μL sensor spot of TAv–GEPII 1.0 immobilized on biotinylated glass slides by a conventional pipette tip. (d) Representative FRET ratio signal of TAv–GEPII 1.0 immobilized on biotinylated glass upon perfusion with different [K+] buffers (0–100 mM; n = 3).