Table I.
Reverse transcription-quantitative PCR primers.
| A, Gene (mouse) | ||
|---|---|---|
|
| ||
| Gene | Forward primer (5′→3′) | Reverse primer (5′→3′) |
| miR-155 | UUAAUGCUAAUUGUGAUAGGGGU | TGTGACCCGAAAGCTCTA |
| ANP | AGGCAGTCGATTCTGCTT | CGTGATAGATGAAGGCAGGAAG |
| BNP | TAGCCAGTCTCCAGAGCAATTC | TTGGTCCTTCAAGAGCTGTCTC |
| Β-MHC | TTGGATGAGCGACTCAAAAA | GCTCCTTGAGCTTCTTCTGC |
| GAPDH | TGCACCACCTGCTTAGC | GGCATGGACTGTGGTCATGAG |
| U6 | GCTTCGGCAGCACATATACTA | CGAATTTGCGTGTCATCCTTG |
|
| ||
| B, Gene (rat) | ||
|
| ||
| Gene | Forward primer (5′→3′) | Reverse primer (5′→3′) |
|
| ||
| miR-155 | AATGCTAATTGTGATAGGGG | GAACATGTCTGCGTATCTC |
| ANP-rat | GAGCAAATCCCGTATACAGTGC | ATCTTCTACCGGCATCTTCTCC |
| BNP-rat | GCTGCTGGAGCTGATAAGAGAA | GTTCTTTTGTAGGGCCTTGGTC |
| Β-MHC | GAGGAGAGGGCGGACATT | ACTCTTCATTCAGGCCCTTG |
| GAPDH | GCAAGAGAGAGGCCCTCAG | TGTGAGGGAGATGCTCAGTG |
| U6 | GGAACGATACAGAGAAGATTAGC | GGAACGATACAGAGAAGATTAGC |
For single-stranded RNA miR-155, its forward primer was based on the sequences of stem-loop and miR-155 using Primer3 Plus (http://www.primer3plus.com/cgi-bin/dev/primer3plus.cgi) and its reverse primer was designed in Primer3 Plus based on the stem-loop sequence and the complementary sequence of miR-155 sequence. miR, microRNA; ANP, atrial natriuretic peptide; BNP, B-type natriuretic peptide; B-MHC, B-myosin heavy chain.