Fig. 6.

CircCRIM1 may encode 188aa protein. CircRNA Db predicted circCRIM1 had ORF with size 188aa (a). 3 × flag sequences were added into putative ORF in original circCRIM1 over-expression plasmid (b). Flag antibody was used to detect the expression of coding protein-3 × flag fusion protein, and there was detectable protein band (c). When the positive control was pulled down, 188aa-3 × flag failed to be pulled down by means of immunoprecipitation with flag antibody (d). CRIM1-188aa and 3 × flag tag were inserted into the protein expression vector and transfected into OVCAR3. Western blot verified the expression (e). CRIM1-188aa over-expression promoted the viability, migration and invasion of OVCAR3 (f, g and h). Three separate experiments were conducted; data are shown as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001