TABLE 1.
Identification of proteins associated with PYR complex DNA-binding activity by MS sequence analysisa
| Band | Identity by MS |
|---|---|
| SWI/SNF subunits | |
| p150 | SRG3 (mouse BAF155) |
| p55a | BAF57 |
| p50 | Actin-related protein, similar to BAF53 |
| p48a | Cytoplasmic actinb |
| p48b | INI1 (BAF47) |
| NuRD subunits | |
| p220 | Mi-2 (CHD4) |
| p58 | HDAC2 |
| p55b | RbAp46 |
| Other | |
| p30 | HMG-1 |
| p22 | DR1 |
a
DNA-affinity column fractions containing peak PYR complex DNA-binding activity were electrophoresed on a 4- to 15%-gradient polyacrylamide gel and electroblotted onto nitrocellulose. Protein bands identified by Ponceau S staining were eluted from the membrane and analyzed by MALDI-ReTOF MS. Bands are named according to their molecular size (e.g., p220 is 220 kDa). Peptides were identified by mass analysis as described in Materials and Methods.
b
Six of six peptides identical to both γ and β actin.