Fig. 2.

High-throughput screening parameters. Vero E6, HEK 293T, A549 and HeLa cells pre-seeded in a white opaque 96-well cell culture plate were either mock infected or infected with rCedV-Luc at MOI 0.01 (A), 0.1 (B) or 1 (C). At 0, 24, 48 and 72 hpi, cells were lysed and relative light units (RLU) in the rCedV-Luc infected cells were measured and normalized by subtracting the signal of mock infected cells from the signal of the rCedV-Luc infected cells. The data represent mean ± standard error from three independent experiments. Luciferase activity at 0 hpi indicate background levels of luminescence. The black dashed line represents the upper limit of detection of the luminometer.