Fig. 9.

Select compounds do not inhibit fusion. CHO-B1 cells in a 96-well plate were transfected with a plasmid encoding one half of a split-luciferase reporter protein. Concurrently CHO-K1 cells in a 6-well were transfected with CedV F and G glycoproteins and with the other half of the split-luciferase. Twenty-four h post transfection the cells were re-suspended and overlaid on the receptor expressing CHO-K1 cells in the 96-well plate. Compounds C1 or F1 were added to a final concentration that ranged between 5 μM to 20 μM. The live-cell luciferase substrate EnduRen was used to monitor the level of cell-cell fusion at the indicated time points. The data represent mean ± standard error from three technical replicates.