Table 3.

Primes and probes for real-time PCR of bacteria.

Items	Primer/probe name and sequence(5ʹ-3ʹ)	Product length/bp
Escherichia coli	DC-F,CATGCCGCGTGTATGAAGAA	96
	DC-R,CGGGTAACGTCAATGAGCAAA
	DC-P,(FMA)AGGTATTAACTTTACTCCCTTCCTC(BHQ-1)
Lactobacillus	RS-F,GAGGCAGCAGTAGGGAATCTTC	126
	RS-R,CAACAGTTACTCTGACACCCGTTCTTC
	RS-P,(FMA)AAGAAGGGTTTCGGCTCGTAAAACTCTGTT(BHQ-1)
Bifidobacterium	SQ-F,CGCGTCCGGTGTGAAAG	121
	SQ-R,CTTCCCGATATCTACACATTCCA
	SQ-P, (FMA) ATTCCACCGTTACACCGGGAA(BHQ-1)
Bacillus	YB-F,GCAACGAGCGCAACCCTTGA	92
	YB-R,TCATCCCCACCTTCCTCCGGT
	YB-P, (FMA)CGGTTTGTCACCGGCAGTCACCT(BHQ-1)
Total bacteria	Eub338F,ACTCCTACGGGAGGCAGCAG	200
	Eub518R,ATTACCGCGGCTGCTGG