Table 2.
Primer sequences and annealing temperature of pigs.
| Target gene | Forward and reverse primer (5ʹ –3ʹ) | Product length | Annealing temperature | Accession number |
|---|---|---|---|---|
| EGF | F:ATCTCAGGAATGGGAGTCAACC R: TCACTGGAGGATGGAATACAGC |
165 | 60 | NM_214020.1 |
| IGF-1 | F:CTGAGGAGGCTGGAGATGTACT R: CCTGAACTCCCTCTACTTGTGTTC |
137 | 60 | NM_001097417.1 |
| Bax | F:AAGCGCATTGGAGATGAACT R: TGCCGTCAGCAAACATTTC |
121 | 60 | XM_013998624.1 |
| Bcl-2 | F:TGCCTTTGTGGAGCTGTATG R: GCCCGTGGACTTCACTTATG |
144 | 60 | XM_003121700.4 |
| SGLT-1 | F:GCAACAGCAAAGAGGAGCGTAT R: GCCACAAAACAGGTCATAGGTC |
137 | 60 | NM_001164021.1 |
| GLUT-2 | F:GACACGTTTTGGGTGTTCCG R: GAGGCTAGCAGATGCCGTAG |
149 | 60 | NM_001097417.1 |
| SLC7A1 | F:TCTTTGCAGGTCGTTTGGGA R: GGCTGATCACCTGTTGGAGT |
137 | 60 | NM_001012613.1 |
| DMT1 | F:GCAGGTGGTTGACGTCTGTA R: CACGCCCCCTTTGTAGATGT |
100 | 60 | NM_001128440.1 |
| ZNT1 | F:TGCTCTGCATGCTGTTACTGA R: TGGAAGGAGTCCGAGAGCAT |
97 | 60 | NM_001139470.1 |
| ZNT2 | F:GAGATGTGATCGTGGTGCTGATG R: CGCCCAGATATGCAGGTTGTGC |
119 | 60 | NM_001139475.1 |
| ZIP4 | F:CAGGGTCATCTGGGAAAGGAAGC R: CCGGCACTCAGGCACATCGTG |
101 | 60 | XM_001925360.3 |
| Occludin | F:CAGGTGCACCCTCCAGATTG R: GGACTTTCAAGAGGCCTGGAT |
110 | 60 | NM_001163647.2 |
| MUC1 | F:GTGCCGCTGCCCACAACCTG R: AGCCGGGTACCCCAGACCCA |
141 | 60 | XM_001926883.5 |
| MUC2 | F:GGTCATGCTGGAGCTGGACAGT R: TGCCTCCTCGGGGTCGTCAC |
181 | 60 | XM_013989745.1 |
| IL-10 | F:CCTGGAAGACGTAATGCCGA R: CACGGCCTTGCTCTTGTTTT |
148 | 60 | NM_214041.1 |
| IL-1β | F:ACGTGCAATGATGACTTTGTCTG R: AGAGCCTTCAGCATGTGTGG |
113 | 60 | NM_214055.1 |
| β-actin | F:TCTGGCACCACACCTTCT R: TGATCTGGGTCATCTTCTCAC |
114 | 60 | DQ178122 |