Figure 3.

Urine biochemical analysis of ACE KO mice untreated with AAI. The ACE KO mice were fed at two, four, six, and eight weeks and sacrificed after another two and four weeks, and the urine samples were collected twice peer weeks with self-made metabolism cages. (a) The urine creatinine was found increased in ACE KO mice at two, four, six, and eight weeks untreated-AAI group. (b) Increased urine albumin was observed in ACE2 KO mice at two, four, six, and eight weeks untreated-AAI group. (c) Remarkably increase in urine protein was also observed in ACE KO mice at two, four, six, and eight weeks untreated-AAI group. All values are expressed as the mean ± SD from each group. ** indicates P < 0.01, compared with the WT group