Figure 4.

Chymase and ACE activity in kidney tissue isolated from ACE KO mice treated with AAI. The peptide was designed for chymase, the biosensor platform was proven, that it was specific for chymase, and the biochemical analysis showed the kidney injury. Experimental flowchart of synthesis of the application in CKD mice model. (a) After the treatment AAI, the chymase activity of mice kidney tissue was detected by self-made AuNPs-peptide probe. About increase in chymase activity was detected in four-week disease development AAI-treated ACE KO mice compared with without AAI. The mice were treated 10 mg/kg/3 days AAI for two and four weeks in ACE KO mice. (b) The kidney tissue ACE activity decreased after the two- and four-week treatment of AAI in ACE KO mice. All values are expressed as the mean ± SD from each group. ** indicates P < 0.01 compared with ACE KO mice group without treating AAI