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. 2021 Nov 10;11:734750. doi: 10.3389/fcimb.2021.734750

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Copyright © 2021 Wang, He, Yin, Wu and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stable expression of pEGFP-C1-CsGRN in human LO2 hepatocytes. LO2 hepatocytes were stably transfected with pEGFP-C1 or pEGFP-C1-CsGRN and selected with G418-containing medium. Cell lines were named as LO2-GFP and LO2-GRN, respectively. (A) Green fluorescence was observed by inverted microscope to determine the efficiency of transfection (scale bar is 50 µm), and the quantification of green fluorescence was measured by Image J software. (B) Relative mRNA levels of CsGRN were quantified by q-PCR. (C) The expression of CsGRN protein were determined by Western blotting. (D) Relative protein levels of CsGRN were quantified by Image J software from three independent experiments. ^** P < 0.01 versus control group, ^*** P < 0.001 versus control group.