Figure 3.

Effects of CsGRN overexpression on the regulation of cell-cycle-related genes expression. (A, B) The mRNA and protein expression of p18, CyclinD1, CyclinD3, CDK6, CDK4, P27, P21, and CDK2 were detected in CsGRN-overexpressed LO2 cells. ^* P < 0.05 versus control group, ^** P < 0.01 versus control group, ^*** P < 0.001 versus control group. (C, D) The mRNA and protein expression of cell-cycle-related genes were detected in recombinant CsGRN protein–treated LO2 cells. ^* P < 0.05 versus PBS group, ^** P < 0.01 versus PBS group, ^*** P<0.001 versus PBS group. ^# P < 0.05 versus MBP group, ^## P < 0.01 versus MBP group, ^### P < 0.001 versus MBP group. The relative mRNA levels of the target genes were determined by q-PCR. The protein expression was determined by Western blot, and the quantification of protein expression was measured by Image J software. Three independent experiments were conducted. NS means NO statistic difference.