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. 2021 Nov 10;11:734750. doi: 10.3389/fcimb.2021.734750

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Copyright © 2021 Wang, He, Yin, Wu and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of migration and invasion in CsGRN overexpression LO2 cells. (A, B) Wound healing scratch assay was used to determine the migration of CsGRN-overexpressed LO2 cells (A) and recombinant CsGRN protein–treated LO2 cells (B). Dotted lines show the edges of the wound over time. (C, D) Transwell assay was used to determine migration and invasion of CsGRN-overexpressed LO2 cells (C) and recombinant CsGRN protein–treated LO2 cells (D). Ten random fields were selected, and the migration and invasion were quantified using Image J software. ^** P < 0.01 versus control group, ^*** P < 0.001 versus control group, ^**** P < 0.0001 versus control group.