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. 2021 Nov 10;11:734853. doi: 10.3389/fonc.2021.734853

Figure 3.

Figure 3

DSF increases PD-L1 expression in vitro. (A) The positive relationship between the mRNA expression of PD-L1 and IRF-7 in breast cancer. (B) Flow cytometry result of surface level of PD-L1 on MDA-MB-231 cell and BT-549 cell treated with different doses of DSF for 48 h. (C) The quantification of relative mean fluorescence intensity (MFI) of PD-L1 on MDA-MB-231 cell and BT-549 cell treated with different doses of DSF for 48 h. (D) PD-L1 protein expression after DSF treatment. MDA-MB-231 and BT-549 cells were treated with different doses of DSF for 48 h, and PD-L1 protein levels were analyzed by Western blotting. (E) Quantitative analysis of PD-L1 expression after different doses of DSF for 48 h through ImageJ intensity measurements. (F) The relative mRNA expression levels of PD-L1 in human MDA-MB-231 cells and BT-549 cells treated with indicated concentration of DSF for 48 h. ***p < 0.001. ns, no statistic significance.