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. 2021 Nov 10;8:768947. doi: 10.3389/fcvm.2021.768947

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Copyright © 2021 Li, Xu, Li, Li, Wang, Chen, Wang, Feng, Yin and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of maslinic acid (MA) on hypoxia/reoxygenation (H/R)-induced cardiomyocyte injury. (A) Chemical structure of MA. (B) The cytotoxicity effects of MA on the H9c2 cells were tested by the Cell Counting Kit-8 (CCK-8). (C) After pretreating with indicated concentrations of MA (5, 10, and 20 μM) and being subjected to H/R procedure, the morphology of H9c2 cells were detected by a light microscope. (D,E) The H9c2 cells injury were measured d by CCK-8 assay and lactate dehydrogenase (LDH) release assay. All the values are representative of at least three independent experiments and expressed as the mean ± SD. *P < 0.05 and **P < 0.01 vs. control group; ^#P < 0.05 and ^##P < 0.01 vs. H/R group.