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. 2021 Nov 10;8:768947. doi: 10.3389/fcvm.2021.768947

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Copyright © 2021 Li, Xu, Li, Li, Wang, Chen, Wang, Feng, Yin and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of MA on cardiomyocyte apoptosis and the expression of apoptosis-related proteins after H/R treatment. (A,B) After various treatments, the apoptosis is measured by Annexin V-PE/7-AAD using flow cytometry and the results are expressed in bar graphs. (C) Western blot was performed to assess the expression of apoptosis-related proteins (Bax, Bcl-2, and cleaved caspase-3) in H9c2 cell under H/R conditions. (D–F) The quantitative analysis of apoptosis-related proteins is shown in bar graphs. All the values are representative of at least three independent experiments and expressed as the mean ± SD. **P < 0.01 vs. control group; ^#P < 0.05 and ^##P < 0.01 vs. H/R group.