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. 2021 Nov 10;8:768947. doi: 10.3389/fcvm.2021.768947

Figure 5.

Figure 5

Maslinic acid attenuated H/R-induced inflammation and apoptosis via regulating HMGB1. (A) Final three-dimensional homology structure predictions of the docking conformation between MA and HMGB1. The sticks are shown for catalytic residues LYS-83 and PRO-102 (purple). The hydrogen bond (dashed yellow lines) distances are 2.2 and 2.7. (B–D) After pretreated with 20 μM of MA or plus rHMGB1, the levels of IL-1β, IL-6 and TNF-α in culture medium were assessed by the specific ELISA analyses under H/R conditions. (E) Western blot was performed to assess the expression of apoptosis-related proteins (Bax, Bcl-2, and cleaved caspase-3) in H9c2 cell under H/R conditions. (F–H) The quantitative analysis of the apoptosis-related proteins is shown in bar graphs. All the values are representative of at least three independent experiments and expressed as the mean ± SD. *P < 0.05 and **P < 0.01 vs. H/R group; #P < 0.05 and ##P < 0.01 vs. H/R + MA20 group.