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. 2021 Nov 10;12:741425. doi: 10.3389/fimmu.2021.741425

Figure 1.

Figure 1

BM-DCs is susceptible for PEDV and supports PEDV replication in vitro. (A) Vero cells and BM-DCs were infected by Vero cell-adapted PEDV strain KB2013-p120 at 1MOI for 24 hours then harvested and subjected with immunofluorescence assay using mice serum raised against recombinant PEDV-N protein and visualized by secondary antibodies. Representing IFA images were selected from at least three independent experiments (hereby and thereafter unless specified). (B) BM-DCs were infected by PEDV-KB2013-p120 strain at 1MOI or 2MOI for 24 hours then harvested for western blot using mice serum raised against recombinant PEDV-N protein. Normal BM-DCs cells without PEDV infection were included as control. The Vero cells infected by PEDV-KB2013-p120 strain at 1MOI for 24 hours then harvested for western blot using mice serum raised against recombinant PEDV-N protein. Normal Vero cells without PEDV infection were included as control. Representing western blot images were selected from at least three independent experiments (hereby and thereafter unless specified). (C) BM-DCs and Vero cells were infected by PEDV-KB2013-p120 strain at 1MOI for 24 hours then harvested by TRizol for absolute quantification of PEDV viral RNA copies using qPCR. Error bars represent variation from at least three independent experiments. Significant differences between indicated groups was marked by ***P < 0.001.