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. 2021 Nov 10;12:741425. doi: 10.3389/fimmu.2021.741425

Figure 2.

Figure 2

PEDV replication inhibit expression of SLA-DR in BM-DCs (A) BM-DCs were infected by Vero cell-adapted PEDV strain KB2013-p120 at 1MOI for 24, 48 and 72 hours then harvested for qPCR analysis for mRNA level of SLA-DRα and SLA-DRβ. Transcript of tubulin were analyzed from the same sample to normalize total RNA input. Error bars represent variation from at least three independent experiments. Significant differences between indicated groups was marked by **P < 0.01; ***P < 0.001. (B) BM-DCs were infected by PEDV-KB2013-p120 strain at 1MOI for 24, 48 and 72 hours then harvested for western blot to evaluate SLA-DRα, SLA-DRβ and PEDV-N protein level using corresponding antibodies. Normal BM-DCs cells without PEDV infection were included as control. Tubulin was probed from the same sample to normalize the total protein load. (C) BM-DCs were infected by PEDV-KB2013-p120 strain 1MOI for 24, 48 and 72 hours then stained with anti-SLA-DR antibody followed by visualization of APC labeled goat anti-mouse IgG. Then the cells were subjected to flow cytometry analysis for evaluating cell surface expression of SLA-DR. BM-DCs without PEDV infection stained with normal mouse IgG as primary antibody were included as primary antibody isotype control. Error bars represent variation of quantification of FACS data from at least three independent experiments. Significant differences between indicated groups was marked by **P < 0.01; or ns, non significant.