Figure 3.

Susceptibility of BM-DCs for PEDV is not strain-specific. (A) BM-DCs were infected by PEDV-KB2013-p120 strain at 0.5, 1 and 2MOI for 48 hours then harvested for western blot to evaluate SLA-DRα, SLA-DRβ and PEDV-N protein. Normal BM-DCs cells without PEDV infection were included as control. Tubulin was probed from the same sample to normalize the total protein load. (B) BM-DCs were infected by Vero cell-adapted PEDV strain KB2013-p120, virulent PEDV strain CH/hubei/2016 (GenBank accession number: KY496315.1), and a field PEDV isolate SXYL-21 for 48 hours. Next, cells were harvested for western blot using mice serum raised against recombinant PEDV-N protein and anti-SLA-DRα Mab. Normal BM-DCs cells without PEDV infection were included as control. Tubulin was probed from the same sample to normalize the total protein load. (C) BM-DCs were infected by PEDV-KB2013-p120 strain at 1 MOI for 24 hours then 10μg LPS was added to the PEDV infected BM-DCs to stimulate the cells for another 24 hours. Next, all cells were harvested for western blot to evaluate SLA-DRα, SLA-DRβ and PEDV-N protein. PEDV infected BM-DCs without LPS stimulation and normal BM-DCs cells were included as controls. Tubulin was probed from the same sample to normalize the total protein load.