Figure 5.

PAMs are susceptible for PEDV. (A) PAMs were infected by Vero cell-adapted PEDV strain KB2013-p120 for 24, 48 and 72 hours. Next, cells were harvested for western blot using mice serum raised against recombinant PEDV-N protein and anti-SLA-DRα Mab. Normal PAMs cells without PEDV infection were included as control. Tubulin was probed from the same sample to normalize the total protein load. (B) PAMs were infected by PEDV strain KB2013-p120 for 24, 48 and 72 hours. Next, cells were harvested by TRizol for qPCR analysis of SLA-DRα and β mRNA level. Normal PAMs cells without PEDV infection were included as control. Error bars represent variation from at least three independent experiments. Significant differences between indicated groups was marked by *P < 0.05; **P < 0.01. or ns, non significant.