Table 1.
List of primers using for plasmids construction.
| Primers | Sequence (5’-3’) | Description |
|---|---|---|
| PEDV-S2-F | ttcctcgagatgagtattaggacag | Cloning of S2 |
| PEDV-S2-R | gtgcggccgcTCACAGATCCTCTTCAGAGATGAGTTTCTGCTCctgcacgtggaccttt | |
| PEDV-M-F | tcctcgagatgtctaacggttcta | Cloning of M |
| PEDV-M-R | gtgcggccgcTCACAGATCCTCTTCAGAGATGAGTTTCTGCTCgactaaatgaagcact | |
| PEDV-N-F | tcctcgagatggcttctgtcagtt | Cloning of N |
| PEDV-N-R | gtgcggccgcTCACAGATCCTCTTCAGAGATGAGTTTCTGCTCatttcctgtgtcgaag | |
| PEDV-E-F | tcctcgagatgctacaattagtga | Cloning of E |
| PEDV-E-R | gtgcggccgcTCACAGATCCTCTTCAGAGATGAGTTTCTGCTCtacgtcaataacagta | |
| PEDV-ORF3-F | tcctcgagatgtttcttggactttttca | Cloning of ORF3 |
| PEDV-ORF3-R | tgcggccgcTCACAGATCCTCTTCAGAGATGAGTTTCTGCTCttcactaattgtagcat | |
| SLA-DRα-promoter-F | GCCTGTCGACGCGTAGAATTCACTCATCTGGCCCGTT | Cloning of SLA-DRα-promoter |
| SLA-DRα-promoter-R | GTGTCAGAAGAATCAAGCTTTGACTGATTAAAATTT | |
| SLA- DRβ-promoter-F | GCCTGTCGACGCGTAGAATTCGTCTGTTTCATACACC | Cloning of SLA-DRβ-promoter |
| SLA-DRβ-promoter-R | GTGTCAGAAGAATCAAGCTTGAGGCACCTGAATTGA |