FIGURE 8.

Synthesis and delivery of fibrinogen to pneumonic airspaces is compromised in hepSTAT3^−/− mice in response to endotoxemia followed by pneumonia (PNA). WT and hepSTAT3^−/− mice were given 18 h i.p. saline, 18 h i.p. LPS, or 18 h i.p. LPS followed by a 24-h challenge with i.t. E. coli (10⁶ CFUs). (A) qRT-PCR was used to determine liver mRNA induction for all three fibrinogen peptide chains. ELISAs were performed to determine fibrinogen concentrations in (B) plasma and (C) BALF from the indicated experimental groups. Data are represented as means with SEM, and significance was determined using a 2-way ANOVA followed by a Sidak test for multiple comparisons (*p < 0.05, **p < 0.01). n = 3–6/group from eight independent experiments.