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. 2021 Nov 10;12:748881. doi: 10.3389/fimmu.2021.748881

Figure 1.

Figure 1

Increased SARS-CoV-2 specific CD4 T cell responses in SARS-CoV-2-infected subjects with OTD. Antigen-specific activation of CD4+ T cells was evaluated in PBMC collected from all patients following 24h stimulation with the peptide megapool obtained from the amino acid sequence of the Spike glycoprotein (MP_S), or from the remainder of the SARS-CoV-2 orfeome (MP_R). DMSO was used as a negative control. (A) FACS plot examples of CD134 and CD137 co-expression on total CD4+ T cells. (B) Frequency of AIM+ (CD134+ CD137+) CD4+ T cells obtained by stimulation with MP_S, (C) MP_R or DMSO negative control is shown for the 3 different groups. (D) Activation of SARS-CoV-2 specific CD4+ T cells, in the presence of MP_S and (E) MP_R megapools, is shown as percentage AIM+ CD4+ T cells after subtraction of the vehicle background in the three cohorts. Dotted black lines indicate cut-off values for positivity. (F) Combined AIM+ CD4+ T cells responses to DMSO-subtracted individual peptide megapools. The non-parametric Wilcoxon matched-pairs signed-rank test was used to compare data within the same group. Statistical comparison between three groups of subjects was assessed by the non-parametric Kruskal-Wallis test followed by Dunn’s multiple comparisons. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.