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. 2021 Dec 1;131(23):e150823. doi: 10.1172/JCI150823

Figure 4. Clonotype representation in the preimmunization and postvaccination repertoires of Env-reactive CD4+ T cells.

Figure 4

(A) CD4+ T cell clones were derived from preimmunization repertoires (V2) of 2 volunteers, 106-009 and 106-039, who showed matching postvaccination responses to ConS peptides (V7). Expressed TRA and TRB gene rearrangements were sequenced from mRNA. (B) Protein-level expression of the corresponding TCR Vβ segments at each time point determined by flow cytometry. Plots are gated on live CD3+ cells. (C) The postvaccination TCR Vβ–defined populations shown in B were isolated by FACS to purity. Expressed TRB gene rearrangements were sequenced from mRNA. Each pie chart segment represents a distinct clonotype and the matching clonotype sequences from A.