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. 2021 Dec 1;131(23):e127171. doi: 10.1172/JCI127171

Figure 2. Delayed onset of septic shock in Mif–/– but not Mif-2–/– mice.

Figure 2

(A) WT, Mif–/–, or Mif-2–/– mice were subjected to CLP (n = 16–19 animals) or sham surgery (n = 13 animals), and survival was monitored for at least 7 days. Log-rank (Mantel-Cox) test was performed to determine significance. (B) Disease score (see Methods) and (C) surface body temperature were recorded in the immediate postoperative period. Two-way ANOVA with Bonferroni’s multiple-comparison test. (D) Creatine kinase (CK) levels and (E) bacterial CFUs determined at 22 hours after CLP or sham surgery in plasma or heparinized whole blood, respectively. Data points are from 5 or more independent experiments and reflect n = 14 to 15 animals per group. For CFUs, each symbol represents 1 animal, n = 6–17 (Mann-Whitney test). (F and G) TNF-α, IL-1α, and CCL2 plasma (F) and peritoneal fluid (G) concentrations in WT, Mif–/–, and Mif-2–/– mice. Cytokine/chemokine levels were measured by flow cytometry using the LEGENDplex Multi Analyte Flow Assay Kit. n = 3 to 4 mice per group, 1-way ANOVA with Tukey’s multiple-comparison test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.