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. 2021 Dec 1;131(23):e127171. doi: 10.1172/JCI127171

Figure 4. MIF deficiency is associated with reduced i.p. SPM numbers.

Figure 4

(A) Representative gating strategy for SPM and LPM analysis in WT, Mif–/–, and Mif-2–/– mice after CLP or sham surgery. Relative cell numbers and absolute cell count analyses of SPMs (B and D) and LPMs (C and E) in the peritoneal lavage of WT, Mif–/–, and Mif-2–/– mice after CLP or sham surgery. Flow cytometric analyses were performed on peritoneal cells collected 22 hours after CLP or sham surgery and included CountBright absolute counting beads in n = 4 to 6 animals/genotype. *P < 0.05; **P < 0.01; ****P < 0.0001 by 1-way ANOVA with Tukey’s multiple-comparison test (B) or Kruskal-Wallis with Dunn’s multiple-comparison test (C–E).