Fig 5. Agarose gel electrophoresis of PCR products of L. martiniquensis and L. orientalis.

A. HSP70-I-3′-UTR PCR products B. SSU-rRNA PCR products C. ITS1-rRNA PCR products. Human blood DNA at 19.8 ng per reaction was used as a background control. N = negative control and lanes 1–10 indicate amounts of 100,000; 10,000; 1,000; 100; 10; 1; 0.1; 0.01; 0.001; and 0.0001 pg of Leishmania DNA in each reaction, respectively.