Table 1. In silico prediction of the HSP70-I-3′-UTR-BsuRI restriction fragments and fragment sizes in PCR-RFLP.

Leishmania species	Isolates	Accession number	PCR product size (bp)	BsuRI restriction fragments	Fragment sizes (bp) by in silico prediction	Fragment sizes (bp) on 3% agarose gel
L. martiniquensis	MHOM/MQ/1992/MAR1;LEM2494	MK607435	482	3	343, 131, 8	ND
	MHOM/TH/2012/LSCM1	MK607436	480	3	341, 131, 8	341, 131
	MHOM/TH/2013/LSCM2	MK60737	482	3	343, 131, 8	ND
	MHOM/TH/2013/LSCM3	MK60738	480	3	341, 131, 8	ND
	MHOM/TH/2015/LSCM5	MK60739	480	3	341, 131, 8	ND
L. orientalis	MHOM/TH/2014/LSCM4	MK607444	674	4	300, 286, 82, 6	300–286a, 82
L. braziliensis	MHOM/GT/2001/U1096	MK607440	562	4	330, 104, 81, 47	330, 104–81a
L. guyanensis	MHOM/BR/1975/M4147	MK607441	627	3	442, 104, 81	>500, 442, 104–81a
L. panamensis	MHOM/PA/1971/LS94	MK607442	629	3	444, 104, 81	>500, 442, 104–81a
L. mexicana	MNYC/BZ/1962/M379	MK607446	738	3	325, 237, 176	325, 237, 176
L. aethiopica	MHOM/ET/1972/L100;LV546	HE575327	742	3	321, 243, 178	321, 243, 178
L. amazonensis	MHOM/BR/1997/M2269	MK607447	740	3	413, 216, 111	413, 216, 111
L. tropica	MHOM/IR/1960/LV357	MK607448	746	3	321, 243, 182	321, 243, 182
L. donovani	MHOM/ET/1967/HU3;LV9	HE575332	753	4	437, 165, 124, 27	437, 165, 124
L. major	MHOM/IL/1980/FV1	MK607449	759	4	323, 188, 178, 70	323, 188–178a, 70
L. infantum	MCAN/ES/1998/JPCM5	MK607450	773	4	436, 182, 128, 27	436, 182, 128

ND = not done

^aMerged band from 2 DNA fragments that were different in size approximately 20 bp or less.