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. 2021 Nov 30;16(11):e0258682. doi: 10.1371/journal.pone.0258682

Fig 3. Evidence for selective PrP co-enrichment of NKAs in mouse brain.

Fig 3

(A) Quantitative mass spectrometry sample processing workflow. (B-D) Side-by-side box plots depicting relative peptide enrichment levels in tryptic digests of PrP brain interactome eluates. (B) Selective enrichment of PrP-derived peptides in wild-type eluates (not in PrP ko eluates). (C) Exemplary PrP co-enrichment of the NKA alpha-1 subunit ATP1A1 (note that other NKA subunits were also observed to co-enrich with PrP; see Fig 2 for details). (D) Immunoglobulin levels were observed at similar abundance levels in all samples, including interactome eluates from PrP ko cells, consistent with the interpretation that levels of the bait antibody were balanced in all samples.