VAT metabolome
of the discordant phenotype of obesity. (A) Fold
changes in the levels of lipid species between obese subjects with
high insulin resistance and nonobese subjects with insulin sensitivity.
Lipids significantly different between groups were marked in a dark
color and with bold text (adjusted p-value < 0.25).
(B) Spearman correlation matrix of the selected lipids with clinical
variables. Adjusted p-values with a cutoff at <0.05
are marked with +. Positive correlations are in blue, and negative
correlations are in red. (C) ROC curves (AUC%, CI 95%) of the multimetabolite
biomarker model to identify the IS obesity metabotype among the obese
population (IS and high IR) or all of the subjects of the study (normal
weight and obesity and IS or high IR). The model was formed by GPE
18:2, GPI 18:0, GPS 18:0, GPC aa 36:1, GPC aa 36:4, and GPC aa 36:5,
selected by the LASSO method. (D) Boxplot of the levels of the individual
metabolites of the multimetabolite biomarker after logarithmic transformation
and Pareto scaling. Abbreviations: AUC, area under the curve; CI,
confidence interval; GPC, glycerophosphatidylcholine; GPE, glycerophosphatidylethanolamine;
GPI, glycerophosphatidylinositol; PS, glycerophosphatidylserine.