Table 2.
Primers used for RT-PCR detection of microbial species.
| Target species tested | Forward primer | Size (bp) |
|---|---|---|
| Reverse primer | ||
| Total bacteriala | CGGCAACGAGCGCAACCC | 130 |
| CCATTGTAGCACGTGTGTAGCC | ||
| B. fibrisolvens b | ACCGCATAAGCGCACGGA | 65 |
| CGGGTCCATCTTGTACCGATAAAT | ||
| S. bovis b | TTCCTAGAGATAGGAAGTTTCTTCGG | 127 |
| ATGATGGCAACTAACAATAGGGGT | ||
| R. amylophilus b | CTGGGGAGCTGCCTGAAT | 100 |
| CATCTGAATGCGACTGGTTG | ||
| P. ruminicola b | GCGAAAGTCGGATTAATGCTCTATG | 78 |
| CCCATCCTATAGCGGTAAACCTTTG | ||
| R. flavefaciens b | CGAACGGAGATAATTTGAGTTTACTTAGG | 132 |
| CGGTCTCTGTATGTTATGAGGTATTACC | ||
| R. albus b | CCCTAAAAGCAGTCTTAGTTCG | 176 |
| CCTCCTTGCGGTTAGAACA | ||
| F. succinogenes b | GGAGCGTAGGCGGAGATTCA | 97 |
| GCCTGCCCCTGAACTATCCA |
R. flavefaciens, Ruminococcus flavefaciens; R. albus, Ruminococcus albus; F. succinogenes, Fibrobacter succinogenes; B. fibrisolvens, Butyrivibrio fibrisolvens; S. bovis, Streptococcus bovis; P. ruminicola, Prevotella ruminicola; R. amylophilus, Ruminobacter amylophilus.
aDenman and Mcsweeney (2009).
bKhafipour et al. (2009).