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. 2021 Nov 11;12:733921. doi: 10.3389/fimmu.2021.733921

Figure 3.

Figure 3

HIF-1α inhibition suppresses immunometabolic activation of monocytes due to recombinant spike protein (rS1). (A) Monocytes increase extracellular acidification response rate (ECAR) when treated with 30 nM rS1, but this is blocked by pre-treatment with chetomin. (B) Quantification of ECAR by area under the curve (AUC). (C) rS1 increase of expression of IL1B, IL6, CXCL8, and TNF is reversed by chetomin pre-treatment. (D) rS1 increase of IL-1β, IL-6, IL-8, and TNFα is reversed by chetomin pre-treatment. *, **, ***: p < 0.05, p < 0.01, p < 0.001 vs. untreated cells. †, ††, †††: p < 0.05, p < 0.01, p < 0.001 vs. rS1-treated cells. N = 7 biological replicates. Bars and error bars are mean ± SEM.