Figure 3.

Autophagy is downregulated in lens fiber cells in gja8b mutant zebrafish. (A and B) Electron micrographs of lens fiber cells (LFC) and lens epithelial cells (LEC) in 36 hpf WT and gja8b mutants. Yellow arrowheads: autophagic vacuoles (AV). Scale bar: 100 nm. B is the number of AVs per area (40 μm²) in the lens of WT and gja8b mutants (n ≥ 60 cells from 6 lenses). (C and D) Immunoblot for endogenous Map1lc3b from WT and gja8b mutant zebrafish. (D) The quantitative analysis of the relative intensity of Map1lc3b-II compared to the Tubg1 (tubulin, gamma 1) control (n = 3 independent experiments). Mean ± SEM, N.S., p > 0.05, *p < 0.05, ****p < 0.0001