Figure 7.

Co-precipitation of PRMT1 with BTG2

Co-immunoprecipitation of CNOT7-TAP and endogenous PRMT1 with YFP-tagged BTG/Tob proteins. HEK293 cells expressing stably CNOT7-TAP were transfected with plasmids expressing YFP-BTG/Tob fusion proteins or only YFP as control. YFP-tagged proteins were precipitated with GFP-Trap magnetic agarose beads and the co-precipitation of endogenous PRMT1 and of CNOT7-TAP was analysed by western blotting. The anti-GFP antibody used for western blot detection weakly cross-reacts with the protein A domain of the TAP tag; hence, CNOT7-TAP is also detected (marked by an asterisk). Note that the molecular weight of YFP-BTG4 is identical to the one of CNOT7-TAP. The correct expression of YFP-BTG4 is demonstrated by the co-precipitation of CNOT7-TAP that is not observed with YFP alone. YFP-BTG4 expression is also shown in Supplementary Fig. 2A