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. 2021 Jun 1;18(12):2450–2465. doi: 10.1080/15476286.2021.1925476

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 8. — The boxC motif is not mediating the interaction of BTG2 with PRMT1

A) Schematic representation of human BTG2, BTG3, Tob1 and their derivatives used in this experiment. The same colour code is used than in Fig. 4A with in addition BTG3 in orange. B) Co-immunoprecipitation of CNOT7-TAP and PRMT1 with BTG2/Tob proteins. HEK293 cells stably expressing CNOT7-TAP were transfected with plasmids expressing GFP-BTG/Tob-HA fusion proteins or empty GFP expression vector. Proteins were precipitated with GFP-Trap magnetic agarose beads and the co-precipitation was analysed by western blotting. CNOT7-TAP is visible after revelation with anti-GFP antibody (marked by an asterisk) because the antibody weakly binds also to the protein A domain of the TAP tag. In this experiment, the CNOT7-TAP signal is not visible in eluates because of the shorter exposition time. A weak co-precipitation with Tob1(APRO) was detected in this experiment but was not reproducible.